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Genetic Homology Between Bacteria in Pulmonary Abscess or Pyothorax and in the Oral Cavity
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A2633 - Genetic Homology Between Bacteria in Pulmonary Abscess or Pyothorax and in the Oral Cavity
Author Block: R. Katsuda1, J. Inubushi2, T. Eguchi3, K. Terada4, R. Kagami1, Y. Fukuda1, Y. Onishi1, K. Hanaoka1, K. Miyake1, Y. Mizumori1, H. Tsukamoto1, S. Sasaki1, T. Kawamura1, Y. Nakahara1; 1Respiratory Medicine, NHO Himeji Medical Center, Himeji, Japan, 2Shizuoka Inovation Center, Sunstar Inc., Takatsuki, Japan, 3Shizuoka Inovation Center, Sunstar Inc., Sunto-gun, Shizuoka, Japan, 4Terada clinic, Himeji, Japan.
Background: The pathogen in pyothorax or pulmonary abscess is thought to be derived from the oral cavity; however it has not been proven genetically. In this study, we examined genetic homology in four cases of pulmonary abscess and four cases of pyothorax. Objectives: DNA homology between oral bacteria and bacteria isolated from pulmonary abscess or pyothorax was examined using a next-generation sequencer. Methods: The subjects were 4 patients with pulmonary abscess and 4 with pyothorax. Oral bacterial flora was obtained by wiping the oral cavity. Pus was collected by percutaneous needle aspiration and cultured. The isolated strain was identified based on 16S rRNA and the whole genome was sequenced using an Illumina Miseq sequencer. The sequence and known genome information of the same bacterial species were used to design 5 sets of strain-specific primers. Total DNA of oral bacterial flora was amplified by PCR using these primers, and the sequence was compared with the sequence of each isolated bacteria and sequences from the International Nucleotide Sequence Database (INSD) to analyze the homology. Results: Streptococcus anginosus group (n=6), Parvimonas micra (n=1), and Fusobacterium nucleatum (n=1) were isolated from pus. In all patients, all amplified sequences in oral bacterial flora completely matched the genome sequence of the isolated strain, but did not perfectly match the genome for the same species in INSD. Conclusions: To our knowledge, this is the first report proving that bacteria isolated from pulmonary abscess or pyothorax are identical to oral bacteria at the strain level based on genetic homology, and suggesting that these bacteria are derived from the oral cavity.
Author Block: R. Katsuda1, J. Inubushi2, T. Eguchi3, K. Terada4, R. Kagami1, Y. Fukuda1, Y. Onishi1, K. Hanaoka1, K. Miyake1, Y. Mizumori1, H. Tsukamoto1, S. Sasaki1, T. Kawamura1, Y. Nakahara1; 1Respiratory Medicine, NHO Himeji Medical Center, Himeji, Japan, 2Shizuoka Inovation Center, Sunstar Inc., Takatsuki, Japan, 3Shizuoka Inovation Center, Sunstar Inc., Sunto-gun, Shizuoka, Japan, 4Terada clinic, Himeji, Japan.
Background: The pathogen in pyothorax or pulmonary abscess is thought to be derived from the oral cavity; however it has not been proven genetically. In this study, we examined genetic homology in four cases of pulmonary abscess and four cases of pyothorax. Objectives: DNA homology between oral bacteria and bacteria isolated from pulmonary abscess or pyothorax was examined using a next-generation sequencer. Methods: The subjects were 4 patients with pulmonary abscess and 4 with pyothorax. Oral bacterial flora was obtained by wiping the oral cavity. Pus was collected by percutaneous needle aspiration and cultured. The isolated strain was identified based on 16S rRNA and the whole genome was sequenced using an Illumina Miseq sequencer. The sequence and known genome information of the same bacterial species were used to design 5 sets of strain-specific primers. Total DNA of oral bacterial flora was amplified by PCR using these primers, and the sequence was compared with the sequence of each isolated bacteria and sequences from the International Nucleotide Sequence Database (INSD) to analyze the homology. Results: Streptococcus anginosus group (n=6), Parvimonas micra (n=1), and Fusobacterium nucleatum (n=1) were isolated from pus. In all patients, all amplified sequences in oral bacterial flora completely matched the genome sequence of the isolated strain, but did not perfectly match the genome for the same species in INSD. Conclusions: To our knowledge, this is the first report proving that bacteria isolated from pulmonary abscess or pyothorax are identical to oral bacteria at the strain level based on genetic homology, and suggesting that these bacteria are derived from the oral cavity.
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