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Role of Gut Microbiome in the Pathogenesis of Allergic Airway Disease
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A5805 - Role of Gut Microbiome in the Pathogenesis of Allergic Airway Disease
Author Block: S. Kim, M. Kang, J. Ha, H. Cho, J. Yoon, C. Kim; Department of Otorhinolaryngology, Yonsei University College of Medicine, Seoul, Korea, Republic of.
Gut microbiota and the host have a symbiotic relationship, whereas altered gut microbiota has been associated with immune-mediated diseases, including allergic airway diseases. The first colonizers are derived from the mother before and during delivery and there is emerging evidence of an early-life critical window, when the effects of gut microbial dysbiosis are most influential in immune development at the mucosal surfaces. Here we sought to further clarify the pathophysiology of allergic airway diseases by determining whether perinatal antibiotic treatment has altered of gut microbiota and regulates susceptibility to a TH2 model of allergic asthma. Allergic asthma was induced BALB/c wild-type mice treated perinatally with ampicillin, vancomycin, and metronidazole in drinking water. Disease severity was assessed by measuring lung inflammation, pathology, cytokine response, and serum antibodies. Microbial community analyses were performed on stool samples via Next Generation Sequencing. We found that perinatal triple antibiotics treatment profoundly altered the gut microbial composition and were not observed to develop phenotype of allergic asthma. Perinatal antigen exposure of mice elicited a splenomegaly that was induced by neutrophils in spleen. Interestingly, we showed a decreased in dendritic cells of lung which reside in asthmatic neonatal group. Our findings will inform the development of novel approaches to investigate the relationship lung-gut axis based on modulating the composition of the gut microbiota.
Author Block: S. Kim, M. Kang, J. Ha, H. Cho, J. Yoon, C. Kim; Department of Otorhinolaryngology, Yonsei University College of Medicine, Seoul, Korea, Republic of.
Gut microbiota and the host have a symbiotic relationship, whereas altered gut microbiota has been associated with immune-mediated diseases, including allergic airway diseases. The first colonizers are derived from the mother before and during delivery and there is emerging evidence of an early-life critical window, when the effects of gut microbial dysbiosis are most influential in immune development at the mucosal surfaces. Here we sought to further clarify the pathophysiology of allergic airway diseases by determining whether perinatal antibiotic treatment has altered of gut microbiota and regulates susceptibility to a TH2 model of allergic asthma. Allergic asthma was induced BALB/c wild-type mice treated perinatally with ampicillin, vancomycin, and metronidazole in drinking water. Disease severity was assessed by measuring lung inflammation, pathology, cytokine response, and serum antibodies. Microbial community analyses were performed on stool samples via Next Generation Sequencing. We found that perinatal triple antibiotics treatment profoundly altered the gut microbial composition and were not observed to develop phenotype of allergic asthma. Perinatal antigen exposure of mice elicited a splenomegaly that was induced by neutrophils in spleen. Interestingly, we showed a decreased in dendritic cells of lung which reside in asthmatic neonatal group. Our findings will inform the development of novel approaches to investigate the relationship lung-gut axis based on modulating the composition of the gut microbiota.
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