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Interaction Between Exposures to Electronic Cigarette Vapors and Cigarette Smoke: Impacts on Pulmonary Leukocyte Populations and Lung Functions
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A7146 - Interaction Between Exposures to Electronic Cigarette Vapors and Cigarette Smoke: Impacts on Pulmonary Leukocyte Populations and Lung Functions
Author Block: A. Lechasseur1, C. Huppé1, J. Routhier1, M. Talbot1, M. Hamel-Auger2, S. Aubin1, M. Beaulieu1, M. Paré1, D. Marsolais1, M. C. Morissette3; 1Quebec Heart and Lung Institute, Quebec, QC, Canada, 2Quebec Heart and Lung Institute, Québec, QC, Canada, 3Quebec Heart and Lung Instiute, Quebec, QC, Canada.
RATIONALE. Over 75% of Canadian electronic cigarette (EC) users are also tobacco cigarette users. Due to the novelty of the product, very little is known on the impact of EC on pulmonary health, even less on dual use of EC and tobacco cigarettes. OBJECTIVES. To investigate the impact of dual exposure to EC and cigarette smoke (CS) on lung functions and leukocyte populations in a mouse model. METHODS. Mice were exposed to the mainstream CS of 3R4F research cigarettes in a whole-body exposure system (SIU24, Promech Lab) 2h/day, 5 days/week for up-to 8 weeks. Mice were also exposed to EC vapors 2h/day, 5 days/week for up-to 8 weeks using our whole-body system and e-liquid containing 30% glycerol and 70% propylene glycol. No flavors or nicotine were added to the e-liquid. CS exposure was performed in the morning and EC exposure in the afternoon. Upon euthanasia, pulmonary functions were assessed using the FlexiVent. Lung leukocytes were assessed in the bronchoalveolar lavage (BAL) by microscopy and in whole-tissue single cell suspension by flow cytometry. RESULTS. Mice subjected to dual exposure for 2 weeks had higher macrophage and neutrophil counts in the BAL than CS-exposed mice. While no differences were observed in the BAL after 8 weeks of exposure, flow cytometry analysis of lung single-cell suspension showed that EC affected the number of macrophages, neutrophils, CD4 T lymphocytes and B lymphocytes. Moreover, dual-exposed and CS-exposed had different proportions of macrophages, CD4 T lymphocytes and B lymphocytes in the tissue. No changes were observed for dendritic cells and CD8 T lymphocytes. Lung functions analysis showed that EC and dual exposure mainly affects tissue resistance, when compared to room air-exposed and CS-exposed mice respectively. CONCLUSIONS. These data suggest that EC vapors, alone or in addition to CS exposure, affect innate and adaptive immune cells. Further investigations will be required to determine if these changes have functional relevance.
Author Block: A. Lechasseur1, C. Huppé1, J. Routhier1, M. Talbot1, M. Hamel-Auger2, S. Aubin1, M. Beaulieu1, M. Paré1, D. Marsolais1, M. C. Morissette3; 1Quebec Heart and Lung Institute, Quebec, QC, Canada, 2Quebec Heart and Lung Institute, Québec, QC, Canada, 3Quebec Heart and Lung Instiute, Quebec, QC, Canada.
RATIONALE. Over 75% of Canadian electronic cigarette (EC) users are also tobacco cigarette users. Due to the novelty of the product, very little is known on the impact of EC on pulmonary health, even less on dual use of EC and tobacco cigarettes. OBJECTIVES. To investigate the impact of dual exposure to EC and cigarette smoke (CS) on lung functions and leukocyte populations in a mouse model. METHODS. Mice were exposed to the mainstream CS of 3R4F research cigarettes in a whole-body exposure system (SIU24, Promech Lab) 2h/day, 5 days/week for up-to 8 weeks. Mice were also exposed to EC vapors 2h/day, 5 days/week for up-to 8 weeks using our whole-body system and e-liquid containing 30% glycerol and 70% propylene glycol. No flavors or nicotine were added to the e-liquid. CS exposure was performed in the morning and EC exposure in the afternoon. Upon euthanasia, pulmonary functions were assessed using the FlexiVent. Lung leukocytes were assessed in the bronchoalveolar lavage (BAL) by microscopy and in whole-tissue single cell suspension by flow cytometry. RESULTS. Mice subjected to dual exposure for 2 weeks had higher macrophage and neutrophil counts in the BAL than CS-exposed mice. While no differences were observed in the BAL after 8 weeks of exposure, flow cytometry analysis of lung single-cell suspension showed that EC affected the number of macrophages, neutrophils, CD4 T lymphocytes and B lymphocytes. Moreover, dual-exposed and CS-exposed had different proportions of macrophages, CD4 T lymphocytes and B lymphocytes in the tissue. No changes were observed for dendritic cells and CD8 T lymphocytes. Lung functions analysis showed that EC and dual exposure mainly affects tissue resistance, when compared to room air-exposed and CS-exposed mice respectively. CONCLUSIONS. These data suggest that EC vapors, alone or in addition to CS exposure, affect innate and adaptive immune cells. Further investigations will be required to determine if these changes have functional relevance.
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