Home
|
Inbox
|
Search
|
View Abstract
Adaptive Immune Response in RSV and RV Infants Hospitalized for Bronchiolitis
Description
.abstract img { width:300px !important; height:auto; display:block; text-align:center; margin-top:10px } .abstract { overflow-x:scroll } .abstract table { width:100%; display:block; border:hidden; border-collapse: collapse; margin-top:10px } .abstract td, th { border-top: 1px solid #ddd; padding: 4px 8px; } .abstract tbody tr:nth-child(even) td { background-color: #efefef; } .abstract a { overflow-wrap: break-word; word-wrap: break-word; }
A2862 - Adaptive Immune Response in RSV and RV Infants Hospitalized for Bronchiolitis
Author Block: A. Frassanito1, R. Nenna1, L. Petrarca1, G. Di Mattia1, A. Pierangeli1, C. Scagnolari1, I. Schiavoni2, G. Fedele2, F. Midulla1; 1Sapienza University of Rome, Rome, Italy, 2Istituto Superiore di Sanità, Rome, Italy.
Rationale: Bronchiolitis is the first cause of hospitalization in infants under 1 year of age. The quality and the quantity of the immune response may be related to the viral etiology and may be a role in the development of respiratory sequelae. The Th1/Th2 pattern of the immune response during bronchiolitis remains unclear. Our aims were to identify the pattern of the immune response in infants hospitalized for bronchiolitis from Respiratory Syncytial Virus (RSV) and Rhinovirus (RV) and to correlate the immunological response to clinical and demographic characteristics of these patients. Methods: During the 2016-2017 season, we studied 57 patients consecutively hospitalized for bronchiolitis who underwent a nasal washing for the detection of 14 respiratory viruses. Only RSV and RV cases were enrolled. Between the third and the seventh day of the disease, peripheral whole blood was obtained and then stimulated with Staphilococcus enterotoxin B (SEB). Intracellular production of IFNγ and IL4 by CD4 and CD8 T cells was analyzed by flow cytometry in both sample [untreated (NT) and stimulated by SEB (SEB)]. Demographic and clinical data were taken from the medical files. Results: 44 RSV and 13 RV samples were analyzed. There wasn’t significative correlation between family history of atopy and asthma and clinical data with IFNγ and IL4 production. IL4 production was higher in RV than in RSV infants in both sample, NT (p=0.012) and after SEB stimulation (p=0.003). IL-4/IFNγ ratio indicated a Th2 pattern in infants with bronchiolitis from RV and a Th1 in infants with bronchiolitis from RSV. Conclusion. Th1/Th2 immune response seems to identify two groups of infants with bronchiolitis: one with risk factors for asthma and with RV infection and one without asthma risk factors and with RSV infection.
Author Block: A. Frassanito1, R. Nenna1, L. Petrarca1, G. Di Mattia1, A. Pierangeli1, C. Scagnolari1, I. Schiavoni2, G. Fedele2, F. Midulla1; 1Sapienza University of Rome, Rome, Italy, 2Istituto Superiore di Sanità, Rome, Italy.
Rationale: Bronchiolitis is the first cause of hospitalization in infants under 1 year of age. The quality and the quantity of the immune response may be related to the viral etiology and may be a role in the development of respiratory sequelae. The Th1/Th2 pattern of the immune response during bronchiolitis remains unclear. Our aims were to identify the pattern of the immune response in infants hospitalized for bronchiolitis from Respiratory Syncytial Virus (RSV) and Rhinovirus (RV) and to correlate the immunological response to clinical and demographic characteristics of these patients. Methods: During the 2016-2017 season, we studied 57 patients consecutively hospitalized for bronchiolitis who underwent a nasal washing for the detection of 14 respiratory viruses. Only RSV and RV cases were enrolled. Between the third and the seventh day of the disease, peripheral whole blood was obtained and then stimulated with Staphilococcus enterotoxin B (SEB). Intracellular production of IFNγ and IL4 by CD4 and CD8 T cells was analyzed by flow cytometry in both sample [untreated (NT) and stimulated by SEB (SEB)]. Demographic and clinical data were taken from the medical files. Results: 44 RSV and 13 RV samples were analyzed. There wasn’t significative correlation between family history of atopy and asthma and clinical data with IFNγ and IL4 production. IL4 production was higher in RV than in RSV infants in both sample, NT (p=0.012) and after SEB stimulation (p=0.003). IL-4/IFNγ ratio indicated a Th2 pattern in infants with bronchiolitis from RV and a Th1 in infants with bronchiolitis from RSV. Conclusion. Th1/Th2 immune response seems to identify two groups of infants with bronchiolitis: one with risk factors for asthma and with RV infection and one without asthma risk factors and with RSV infection.
|
Home
|
Inbox
|
Search
|