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Does Physical Exercise Influence the Morphofunctionality of Diabetic Rat Lung?
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A2376 - Does Physical Exercise Influence the Morphofunctionality of Diabetic Rat Lung?
Author Block: W. A. Zin1, M. D. Moreira-Gomes2, L. M. Machado2, D. S. Serra3, T. G. Neves4, J. H. Leal-Cardoso2, F. S. Cavalcante3; 1Institute of Biophysics, Federal Univ of Rio De Janeiro, Rio de Janeiro, Brazil, 2Institute of Biomedical Sciences, State University of Ceara, Fortaleza, Brazil, 3Science and Technology Center, State University of Ceara, Fortaleza, Brazil, 4Institute of Biomedical Sciences, State University of Ceara, Ceara, Brazil.
Rationale: Diabetes mellitus (DM) is a metabolic disorder characterized mainly by hyperglycemia and insufficient insulin secretion or tissue resistance to insulin. The lung can be considered as a target organ in DM. Physical exercise can offer DM-specific health benefits, since it is able to decrease glycemia, insulin resistance, and improve glucose tolerance. Thus, we aimed to study the respiratory changes in a murine model of DM and its interaction with physical exercise.
Methods: Our Institutional Committee on Ethics in the Use of Animals (CEUA-UECE) approved the study (0721784/2016). On the 5th day of life male and female Wistar rats received a single dose of streptozotocin (n5-STZ, 120 mg/kg, ip, diabetic group) diluted in sodium citrate (0.1 M, 0.1 mL) or only sodium citrate (control group). Starting on the 6th week, 23 animals ran 5 days/week/9 weeks on a motorized treadmill, and 18 rats remained sedentary, thus forming the control (C, n=11), trained (T, n=15), diabetic (D, n=7) and diabetic trained (DT, n=8) groups. Blood glucose level was measured weekly during the experimental period. In vivo respiratory mechanics [Newtonian resistance (RN), tissue viscance (G) and elastance (H)] were determined. Lungs were prepared for histopathology. ANOVA was used to test differences among groups.
Results: RN, G and H were significantly higher in D mice than in C, T and DT groups (0.082 ± 0.003, 0.059 ± 0.006, 0.055 ± 0.001 and 0.054 ± 0.003 cmH2O.s/mL; 1.00 ± 0.04, 0.46 ± 0.01, 0.52 ± 0.01 and 0.53 ± 0.02 cmH2O/mL; 3.56 ± 0.18, 2.14 ± 0.05, 2.11 ± 0.07 and 2.18 ± 0.09 cmH2O/mL, respectively). The lung inflammatory cell content and bronchoconstriction indexes were significantly higher in D animals than in C, T and DT rats (2.47 ± 0.03, 2.11 ± 0.01, 1.98 ± 0.02 and 2.05 ± 0.04 cells-2/µm2; 8.07 ± 0.7, 4.23 ± 0.2, 3.67 ± 0.1 and 4.62 ± 0.1). Alveolar collapse was higher in D (10.23%) than in C (8.03%), T (8%) and DT (7.57%). The glycemia was significantly larger in D than in C, T and DT (190.8 ± 21.7, 102.9 ± 2.6, 81.7 ± 3.0 e 96.0 ± 4.0 mg/dL, respectively).
Conclusion: Physical exercise is able to prevent functional and histological changes in the respiratory system and decrease blood glycemia in diabetic rats.
Author Block: W. A. Zin1, M. D. Moreira-Gomes2, L. M. Machado2, D. S. Serra3, T. G. Neves4, J. H. Leal-Cardoso2, F. S. Cavalcante3; 1Institute of Biophysics, Federal Univ of Rio De Janeiro, Rio de Janeiro, Brazil, 2Institute of Biomedical Sciences, State University of Ceara, Fortaleza, Brazil, 3Science and Technology Center, State University of Ceara, Fortaleza, Brazil, 4Institute of Biomedical Sciences, State University of Ceara, Ceara, Brazil.
Rationale: Diabetes mellitus (DM) is a metabolic disorder characterized mainly by hyperglycemia and insufficient insulin secretion or tissue resistance to insulin. The lung can be considered as a target organ in DM. Physical exercise can offer DM-specific health benefits, since it is able to decrease glycemia, insulin resistance, and improve glucose tolerance. Thus, we aimed to study the respiratory changes in a murine model of DM and its interaction with physical exercise.
Methods: Our Institutional Committee on Ethics in the Use of Animals (CEUA-UECE) approved the study (0721784/2016). On the 5th day of life male and female Wistar rats received a single dose of streptozotocin (n5-STZ, 120 mg/kg, ip, diabetic group) diluted in sodium citrate (0.1 M, 0.1 mL) or only sodium citrate (control group). Starting on the 6th week, 23 animals ran 5 days/week/9 weeks on a motorized treadmill, and 18 rats remained sedentary, thus forming the control (C, n=11), trained (T, n=15), diabetic (D, n=7) and diabetic trained (DT, n=8) groups. Blood glucose level was measured weekly during the experimental period. In vivo respiratory mechanics [Newtonian resistance (RN), tissue viscance (G) and elastance (H)] were determined. Lungs were prepared for histopathology. ANOVA was used to test differences among groups.
Results: RN, G and H were significantly higher in D mice than in C, T and DT groups (0.082 ± 0.003, 0.059 ± 0.006, 0.055 ± 0.001 and 0.054 ± 0.003 cmH2O.s/mL; 1.00 ± 0.04, 0.46 ± 0.01, 0.52 ± 0.01 and 0.53 ± 0.02 cmH2O/mL; 3.56 ± 0.18, 2.14 ± 0.05, 2.11 ± 0.07 and 2.18 ± 0.09 cmH2O/mL, respectively). The lung inflammatory cell content and bronchoconstriction indexes were significantly higher in D animals than in C, T and DT rats (2.47 ± 0.03, 2.11 ± 0.01, 1.98 ± 0.02 and 2.05 ± 0.04 cells-2/µm2; 8.07 ± 0.7, 4.23 ± 0.2, 3.67 ± 0.1 and 4.62 ± 0.1). Alveolar collapse was higher in D (10.23%) than in C (8.03%), T (8%) and DT (7.57%). The glycemia was significantly larger in D than in C, T and DT (190.8 ± 21.7, 102.9 ± 2.6, 81.7 ± 3.0 e 96.0 ± 4.0 mg/dL, respectively).
Conclusion: Physical exercise is able to prevent functional and histological changes in the respiratory system and decrease blood glycemia in diabetic rats.
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