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IL-9 Deficiency Promotes Th17 Response in a Murine Model of Pneumocystis Pneumonia
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A4699 - IL-9 Deficiency Promotes Th17 Response in a Murine Model of Pneumocystis Pneumonia
Author Block: T. Li, Z. Tong; Beijing Chaoyang Hospital Capital Medical University, Beijing, China.
Rationale: Pneumocystis is an opportunistic fungal pathogen which causes life-threatening pneumonia in immunocompromised patients. Previous studies demonstrate a protective CD4+ T cells dependent immune response in the defense of Pneumocystis. The role of T helper 9 (Th9) cells in Pneumocystis pneumonia (PCP) remains unknown. IL-17/Th17 is reported to play a role in the control of a variety of fungal infection, and is observed to accelerate the clearance of Pneumocystis in mice model. Whether IL-9 is associated with IL-17 in Pneumocystis infection deserves to be explored.Methods: Using IL-9 deficient (IL-9-/-) mice, flow cytometry and real-time PCR were conducted to investigate the immune function related to Th17 response in defense against Pneumocystis infection. At the same time, the differentiation to Th17 cells were detected using CD4+ naïve T cells from IL-9-/- and WT mice spleens.Results: It was observed that reduced Pneumocystis burden was found in lungs at 3 week post infection compared with wild-type (WT) mice in IL-9-/- PCP mice. Compared with WT mice, IL-9-/- mice exhibited stronger Th17 immune responses by flow cytometer and real-time PCR. ELISA revealed higher level of IL-17 in BALF from IL-9-/- mice than WT mice. And IL-9 deficiency promoted Th17 differentiation from CD4+ naïve T cells in vitro. Conclusion: Taken together, these results demonstrated that IL-9 deficiency reduced the Pneumocystis burden and promoted the pulmonary Th17 response during Pneumocystis infection, probably via enhancing the differentiation and migration of Th17 cells.
Author Block: T. Li, Z. Tong; Beijing Chaoyang Hospital Capital Medical University, Beijing, China.
Rationale: Pneumocystis is an opportunistic fungal pathogen which causes life-threatening pneumonia in immunocompromised patients. Previous studies demonstrate a protective CD4+ T cells dependent immune response in the defense of Pneumocystis. The role of T helper 9 (Th9) cells in Pneumocystis pneumonia (PCP) remains unknown. IL-17/Th17 is reported to play a role in the control of a variety of fungal infection, and is observed to accelerate the clearance of Pneumocystis in mice model. Whether IL-9 is associated with IL-17 in Pneumocystis infection deserves to be explored.Methods: Using IL-9 deficient (IL-9-/-) mice, flow cytometry and real-time PCR were conducted to investigate the immune function related to Th17 response in defense against Pneumocystis infection. At the same time, the differentiation to Th17 cells were detected using CD4+ naïve T cells from IL-9-/- and WT mice spleens.Results: It was observed that reduced Pneumocystis burden was found in lungs at 3 week post infection compared with wild-type (WT) mice in IL-9-/- PCP mice. Compared with WT mice, IL-9-/- mice exhibited stronger Th17 immune responses by flow cytometer and real-time PCR. ELISA revealed higher level of IL-17 in BALF from IL-9-/- mice than WT mice. And IL-9 deficiency promoted Th17 differentiation from CD4+ naïve T cells in vitro. Conclusion: Taken together, these results demonstrated that IL-9 deficiency reduced the Pneumocystis burden and promoted the pulmonary Th17 response during Pneumocystis infection, probably via enhancing the differentiation and migration of Th17 cells.
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