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Potential of the Phage Depolymerase from a Myoviridae Bacteriophage vB_AbaM_IME200 Against Pandrug-Resistant Acinetobacter Baumannii
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A5488 - Potential of the Phage Depolymerase from a Myoviridae Bacteriophage vB_AbaM_IME200 Against Pandrug-Resistant Acinetobacter Baumannii
Author Block: C. Bai1, Y. Liu1, Z. Mi2, Y. Tong2; 1Department of Respiratory and Critical Care Diseases, 307th Hospital of PLA, Beijing, China, 2State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China.
RATIONALE: The emergence of multidrug- or pandrug-resistant Acinetobacter baumannii (A. baumannii) has made it difficult to treat and control infections caused by this bacterium. METHODS: A novel Myoviridae bacteriophage was isolated from hospital sewage using a pandrug-resistant A. baumannii strain as the host bacterium, and its lytic ability, genomic compositions were studied. Meanwhile, the phage depolymerase was identified and its contribution to bactericidal activity was determined. RESULTS: The results showed that the phage developed clear plaques surrounded by enlarged halos on the host bacterium. Moreover, the phage lysed ten of 41 strains of A. baumannii and had a latent period of 10 min and a burst size of about 229 plaque-forming units per cell. Genomic analysis indicated that the phage had a double-stranded genome of 41,243 bp, with a G+C content of 39.3 %. An Open Reading Frame annotated with encoding the tail fiber protein was proved to be responsible for halo development, namely express a protein with depolymerase activity. Results of in vitro bactericidal assay indicated that combination of the phage depolymerase and serum effectively reduce counts of pandrug-resistant A. baumannii, and amino acid sequence alignment showed that depolymerases derived from A. baumannii phages had a relatively short highly conserved domain in their N-terminal sequence, but their amino acid sequences were highly diverse in C-terminal sequence. CONCLUSION: This phage and its depolymerase had strong lytic ability against pandrug-resistant A. baumannii and may have potential applications in future treatment to pandrug-resistant bacterial infections.
Author Block: C. Bai1, Y. Liu1, Z. Mi2, Y. Tong2; 1Department of Respiratory and Critical Care Diseases, 307th Hospital of PLA, Beijing, China, 2State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China.
RATIONALE: The emergence of multidrug- or pandrug-resistant Acinetobacter baumannii (A. baumannii) has made it difficult to treat and control infections caused by this bacterium. METHODS: A novel Myoviridae bacteriophage was isolated from hospital sewage using a pandrug-resistant A. baumannii strain as the host bacterium, and its lytic ability, genomic compositions were studied. Meanwhile, the phage depolymerase was identified and its contribution to bactericidal activity was determined. RESULTS: The results showed that the phage developed clear plaques surrounded by enlarged halos on the host bacterium. Moreover, the phage lysed ten of 41 strains of A. baumannii and had a latent period of 10 min and a burst size of about 229 plaque-forming units per cell. Genomic analysis indicated that the phage had a double-stranded genome of 41,243 bp, with a G+C content of 39.3 %. An Open Reading Frame annotated with encoding the tail fiber protein was proved to be responsible for halo development, namely express a protein with depolymerase activity. Results of in vitro bactericidal assay indicated that combination of the phage depolymerase and serum effectively reduce counts of pandrug-resistant A. baumannii, and amino acid sequence alignment showed that depolymerases derived from A. baumannii phages had a relatively short highly conserved domain in their N-terminal sequence, but their amino acid sequences were highly diverse in C-terminal sequence. CONCLUSION: This phage and its depolymerase had strong lytic ability against pandrug-resistant A. baumannii and may have potential applications in future treatment to pandrug-resistant bacterial infections.
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