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Airway Surface Liquid Antimicrobial Activity Follows a Seasonal Pattern
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A7240 - Airway Surface Liquid Antimicrobial Activity Follows a Seasonal Pattern
Author Block: L. G. Vargas Buonfiglio1, O. G. Vanegas Calderon2, A. K. Gerke1, P. Polgreen1, J. Zabner1, A. P. Comellas1; 1Internal Medicine, University of Iowa, Iowa City, IA, United States, 2Pediatrics, University of Iowa, Iowa City, IA, United States.
Rationale: Airway infections follow a seasonal pattern characterized by increased infections during winter compared to summer. One proposed mechanism is that during winter there is more prevalence of vitamin D3 deficiency due to a decrease in total sun exposure. The airway surface liquid (ASL) antimicrobial activity is part of the innate immune mechanism that prevents airway infections. Many antimicrobial peptides in the ASL are upregulated by vitamin D3. We hypothesized that the ASL antimicrobial activity will be greater during the summer compared to the winter months.
Methods: 40 voluntaries from Iowa underwent bronchoscopies with ASL collection between January and December of 2008-2009. We performed an analysis of the antimicrobial activity of human ASL collected via bronchoscopy against ~5x105 CFU of a bioluminescent S. aureus. We interpreted a reduction in Relative Light Units (RLU) at 4 minutes as ASL antimicrobial activity. We grouped the subjects by months when the ASL was collected and plotted the average of RLU along with the total hours of sunshine for the state of Iowa. In parallel, serum concentration of active vitamin D3 (1,25(OH)2D3) were measured.
Results: We found that the ASL antimicrobial activity gradually changed following a seasonal pattern. In addition, we found that both ASL antimicrobial activity and levels of 1,25(OH)2D3 were significantly higher in the summer (June -August) compared to the winter time (December-February). This is the first report that suggests that the airway may have an intrinsic seasonal antimicrobial activity. We proposed that levels of vitamin D3 may play a role in this effect. Additional studies must determine other alternative mechanisms responsible for this phenomenon.
Author Block: L. G. Vargas Buonfiglio1, O. G. Vanegas Calderon2, A. K. Gerke1, P. Polgreen1, J. Zabner1, A. P. Comellas1; 1Internal Medicine, University of Iowa, Iowa City, IA, United States, 2Pediatrics, University of Iowa, Iowa City, IA, United States.
Rationale: Airway infections follow a seasonal pattern characterized by increased infections during winter compared to summer. One proposed mechanism is that during winter there is more prevalence of vitamin D3 deficiency due to a decrease in total sun exposure. The airway surface liquid (ASL) antimicrobial activity is part of the innate immune mechanism that prevents airway infections. Many antimicrobial peptides in the ASL are upregulated by vitamin D3. We hypothesized that the ASL antimicrobial activity will be greater during the summer compared to the winter months.
Methods: 40 voluntaries from Iowa underwent bronchoscopies with ASL collection between January and December of 2008-2009. We performed an analysis of the antimicrobial activity of human ASL collected via bronchoscopy against ~5x105 CFU of a bioluminescent S. aureus. We interpreted a reduction in Relative Light Units (RLU) at 4 minutes as ASL antimicrobial activity. We grouped the subjects by months when the ASL was collected and plotted the average of RLU along with the total hours of sunshine for the state of Iowa. In parallel, serum concentration of active vitamin D3 (1,25(OH)2D3) were measured.
Results: We found that the ASL antimicrobial activity gradually changed following a seasonal pattern. In addition, we found that both ASL antimicrobial activity and levels of 1,25(OH)2D3 were significantly higher in the summer (June -August) compared to the winter time (December-February). This is the first report that suggests that the airway may have an intrinsic seasonal antimicrobial activity. We proposed that levels of vitamin D3 may play a role in this effect. Additional studies must determine other alternative mechanisms responsible for this phenomenon.
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