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A7722 - Tracheal Replacement Using a Bio-3D Printed Scaffold-Free Engineered Tissue Based Trachea
Author Block: K. Matsumoto1, R. Machino2, D. Taniguchi3, T. Tetsuo1, Y. Takeoka1, Y. Takeoka1, S. Oyama1, M. Moriyama1, Y. Taura1, N. Yamasaki1, N. Yamasaki1, K. Takagi2, T. Tsuchiya2, T. Miyazaki2, G. Hatachi2, K. Tomoshige1, K. Nakayama4, K. Nakayama4, T. Nagayasu1; 1Surgical Oncology, Nagasaki University, Nagasaki, Japan, 2Nagasaki University, Nagasaki, Japan, 3Department of Surgical Oncology,, Nagasaki Universi, Nagasaki, Japan, 4Saga University, Saga, Japan.
Tracheal replacement by scaffold-free tissue engineered trachea made by bio-3D printer
Keitaro Matsumoto1,2, Ryusuke Machino1, Daisuke Taniguchi1,2, Tomoyuki Tesuo1,2, Yosuke Takeoka1,2, Shozaburo Oyama1,2, Masaaki Moriyama1,2, Yasuyuki Taura1, Naoya Yamasaki1,2, Katsunori Tagagi1,2, Tomoshi Tsuchiya1, Takuro Miyazaki1, Go Hatachi1,2, Koichi Tomoshige1,2, Koichi Nakayama3, Takeshi Nagayasu1,2
1 Department of Surgical Oncology, Nagasaki University Graduate School of Biomedical Sciences
2 Medical-engineering hybrid professional development center, Nagasaki University Graduate School of Biomedical Sciences
3 BioMedical Engineering Course Advanced Technology Fusion Graduate School of Science and Engineering, Saga University
1-7-1 Sakamoto, Nagasaki, 8528501, Japan
TEL 095-819-7304
FAX 095-819-7306
Abstract
Introduction: Most artificial airway organs with scaffolds have some limitations, such as inflexibility in design, lack of cytocompatibility, toxic effects, and post-transplant degradation. The present study sought to generate scaffold-free trachea-like structures using several types of human cells by a bio-three-dimensional (3D) printing system. The rationale of the present study was to generate a scaffold-free trachea-like structure using bio-three-dimensional (bio-3D) printing technology and to assess circumferential tracheal replacement using the structure.
Methods: human cartilage cells (NHACs), human fibroblasts (NHDFs), Human Umbilical Vein Endothelial Cells (HUVECs) and human mesenchymal stem cells from bone marrow (MSCs) were used. By using bio-3D printer “Regenova®”, three types of structure with different combinations of cells were made, such as fibrous structure (NHDFs + HUVECs + MSCs), cartilaginous structure (NHACs + HUVECs + MSCs) and ladder shape structure (alternately built with fibrous structure and cartilaginous structure). The mechanical strength of the artificial trachea and histological and immunohistochemical examinations were performed. The structures were transplanted into F344 rats with stent and immunosuppressant.
Results: The average tensile strengths before transplantation were 448.0 ± 32.0mN, 460.5 ± 294.1mN, 683.0 ± 259.3mN and 832.5 ± 117.4mN in fibrous structure, cartilaginous structure, ladder shape structure and native trachea, respectively. We decided to use ladder shape structures for transplantation. The ladder shaped artificial trachea had sufficient strength to transplant into the trachea with silicone stents, which were used to prevent collapse of the artificial trachea and to support the graft until sufficient blood supply was obtained. The rats survived up to 35days after transplantation. Epithelium extended into the structure from native trachea and covered almost of inner surface of the structure 35 days after transplantation. Chondrogenesis and vasculogenesis were observed, however, they were not sufficient histologically.
Conclusions: The scaffold-free ladder shaped artificial tracheas made by a bio-3D printer could be utilized as trachea grafts in rats.