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Exosome RNA-Seq Analysis to Isolate Lung Cancer Specific Pseudogenes for Liquid Biopsy
Description
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A7078 - Exosome RNA-Seq Analysis to Isolate Lung Cancer Specific Pseudogenes for Liquid Biopsy
Author Block: X. Liu, R. Kiefl, R. M. Huber; Respiratory Medicine and Thoracic Oncology, University of Munich, Munich, Germany.
Rationale:
Recently, it has been reported that several exosome non-coding RNAs are overexpressed in non-small-cell lung cancer (NSCLC), and have been identified as key regulators in post-genomic biology, moreover it also has been suggested as potential biomarkers for a non-invade “liquid biopsy” in cancers’ diagnosis and prognosis. In this study, we screened for human NSCLC associated pseudogenes in plasma exosomes.
Methods:
13 lung adenocarcinoma cancer patients and 15 health volunteers were recruited. We characterized non-coding RNA transcripts by using RNA-seq analyses of ribosomal RNA-depleted total RNA from the plasma exosomes. DESeq2 package was used to identify differentially expressed non-coding RNAs after raw data filtering. The patients’ exosome pseudogenes were enriched at least 2-fold compared with normal control.
Results:
An expression of eight pseudogenes were found to be associated with lung adenocarcinoma cancer patients.
Conclusions:
This study indicates that the pseudogenes can be detected in cancer patients’ plasma exosomes, and eight-pseudogenes signature may serve as an effective non-invade “liquid biopsy” prognostic molecular biomarkers in NSCLC patient.
Although, compared with protein-coding genes, we know less about pseudogenes. The research of pseudogenes is expanding quickly. Therefore, more functional investigations are needed.
Author Block: X. Liu, R. Kiefl, R. M. Huber; Respiratory Medicine and Thoracic Oncology, University of Munich, Munich, Germany.
Rationale:
Recently, it has been reported that several exosome non-coding RNAs are overexpressed in non-small-cell lung cancer (NSCLC), and have been identified as key regulators in post-genomic biology, moreover it also has been suggested as potential biomarkers for a non-invade “liquid biopsy” in cancers’ diagnosis and prognosis. In this study, we screened for human NSCLC associated pseudogenes in plasma exosomes.
Methods:
13 lung adenocarcinoma cancer patients and 15 health volunteers were recruited. We characterized non-coding RNA transcripts by using RNA-seq analyses of ribosomal RNA-depleted total RNA from the plasma exosomes. DESeq2 package was used to identify differentially expressed non-coding RNAs after raw data filtering. The patients’ exosome pseudogenes were enriched at least 2-fold compared with normal control.
Results:
An expression of eight pseudogenes were found to be associated with lung adenocarcinoma cancer patients.
Conclusions:
This study indicates that the pseudogenes can be detected in cancer patients’ plasma exosomes, and eight-pseudogenes signature may serve as an effective non-invade “liquid biopsy” prognostic molecular biomarkers in NSCLC patient.
Although, compared with protein-coding genes, we know less about pseudogenes. The research of pseudogenes is expanding quickly. Therefore, more functional investigations are needed.
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